3UZB
Crystal Structures of Branched-Chain Aminotransferase from Deinococcus radiodurans Complexes with alpha-Ketoisocaproate and L-Glutamate Suggest Its Radio-Resistance for Catalysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.110, 172.580, 80.080 |
| Unit cell angles | 90.00, 108.19, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 2COJ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.375 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.060 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 0.390 |
| Number of reflections | 29754 | |
| <I/σ(I)> | 8.85 | 2.46 |
| Completeness [%] | 87.7 | 87.7 |
| Redundancy | 4.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.15M magnesium chloride, 15%(w/v) polyethylene glycol 8000, 0.1M Tris(pH 7.3), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
