3UYU
Structural basis for the antifreeze activity of an ice-binding protein (LeIBP) from Arctic yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98227 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 97.580, 97.580, 106.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.590 - 1.570 |
| R-factor | 0.21774 |
| Rwork | 0.216 |
| R-free | 0.25073 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.257 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.660 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 71520 | |
| Completeness [%] | 99.4 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 292 | 0.1M sodium acetate, pH 4.5, 1.2M ammonium sulfate, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
