3UXO
Crystal Structure of Rat DNA Polymerase Beta Mutator I260Q Apoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.908, 67.400, 82.456 |
Unit cell angles | 90.00, 115.81, 90.00 |
Refinement procedure
Resolution | 24.046 - 2.100 |
R-factor | 0.2601 |
Rwork | 0.257 |
R-free | 0.31800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.195 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (molecular replacement) |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.590 | 3.280 | 2.190 |
High resolution limit [Å] | 2.077 | 2.940 | 2.080 |
Rmerge | 0.097 | 0.594 | |
Number of reflections | 45730 | ||
<I/σ(I)> | 6.5 | 1.3 | |
Completeness [%] | 99.5 | 99.5 | 99.7 |
Redundancy | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 296 | PEG 3350, MES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 296K |