3UXG
Crystal structure of RFXANK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2011-07-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97911 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 43.598, 98.354, 99.358 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.860 - 1.850 |
R-factor | 0.1883 |
Rwork | 0.187 |
R-free | 0.22150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3so8 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.476 |
Data reduction software | XDS |
Data scaling software | SCALA (CCP4_3.3.16) |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.180 | 49.180 | 1.950 |
High resolution limit [Å] | 1.850 | 5.850 | 1.850 |
Rmerge | 0.120 | 0.050 | 0.880 |
Total number of observations | 4124 | 19184 | |
Number of reflections | 18636 | ||
<I/σ(I)> | 15.0071 | ||
Completeness [%] | 100.0 | 99.08 | 99.97 |
Redundancy | 7.11 | 6.3 | 7.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% PEG3350, 0.2M sodium chloride, 0.1M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% PEG3350, 0.2M sodium chloride, 0.1M HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 291K |