3UXA
Designed protein KE59 R1 7/10H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-12-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 |
| Unit cell lengths | 61.860, 61.860, 119.435 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.465 - 2.500 |
| R-factor | 0.21587 |
| Rwork | 0.212 |
| R-free | 0.28358 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uyc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.648 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.465 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.159 | 0.700 |
| Number of reflections | 17470 | |
| <I/σ(I)> | 9.7 | 2.2 |
| Completeness [%] | 98.3 | 99.4 |
| Redundancy | 3.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.2 M ammonium chloride, 0.1 M sodium acetate, pH 5.0, 20% PEG6000, 3-(1-Methylpiperidinum)-1-propane-sulfonate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
