3UX8
Crystal structure of UvrA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.653, 51.330, 112.395 |
| Unit cell angles | 90.00, 97.27, 90.00 |
Refinement procedure
| Resolution | 37.080 - 2.100 |
| R-factor | 0.2122 |
| Rwork | 0.210 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.257 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.056 | 0.030 | 0.400 |
| Number of reflections | 42002 | ||
| <I/σ(I)> | 15.2 | ||
| Completeness [%] | 98.6 | 92.8 | 98.8 |
| Redundancy | 3.3 | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | BICINE, PEG 20,000, 1,4-dioxane, pH 9.0, vapor diffusion, hanging drop, temperature 277K |
