3UX3
Crystal Structure of Domain-Swapped Fam96a minor dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.179, 52.246, 65.893 |
Unit cell angles | 90.00, 104.56, 90.00 |
Refinement procedure
Resolution | 34.241 - 1.800 |
R-factor | 0.2027 |
Rwork | 0.200 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ux2 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.528 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.776 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.510 |
Number of reflections | 29996 | |
<I/σ(I)> | 18.06 | 2.8 |
Completeness [%] | 99.6 | 100 |
Redundancy | 6.8 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.7 | 278 | sodium acetate, sodium malonate, DMSO, PEG3350, zinc zcetate, pH 4.7, VAPOR DIFFUSION, temperature 278K |