3UWX
Crystal structure of UvrA-UvrB complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 216.823, 216.823, 116.802 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.065 - 4.398 |
R-factor | 0.2906 |
Rwork | 0.288 |
R-free | 0.34860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.793 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.560 |
High resolution limit [Å] | 4.398 | 9.460 | 4.400 |
Rmerge | 0.101 | 0.037 | 0.962 |
Number of reflections | 17635 | ||
<I/σ(I)> | 6.4 | ||
Completeness [%] | 96.6 | 97.6 | 82.7 |
Redundancy | 8.7 | 8.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | Na/K phosphate, PEG 1000, NaCl, trehalose, pH 8.5, vapor diffusion, sitting drop, temperature 295K |