3UWT

Crystal structure of a RNA binding domain of poly-U binding splicing factor 60KDa (PUF60) from Homo sapiens at 2.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537,0.9796,0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	64.173, 64.173, 80.337
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.798 - 2.500
R-factor	0.2042
Rwork	0.203
R-free	0.23990
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.798	29.798	2.570
High resolution limit [Å]	2.500	11.180	2.500
Rmerge		0.014
Total number of observations		508	3532
Number of reflections	6937
<I/σ(I)>	11.4	13.9	1.6
Completeness [%]	99.8	94.7	99.9
Redundancy	6.5	5.5	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	0.2M sodium chloride, 1.26M ammonium sulfate, 0.1M CHES pH 9.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K