3UWN
The 3-MBT repeat domain of L3MBTL1 in complex with a methyl-lysine mimic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Detector technology | CCD |
Collection date | 2011-09-28 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.774, 94.530, 58.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.580 - 2.150 |
R-factor | 0.2327 |
Rwork | 0.231 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oyx |
RMSD bond length | 0.013 |
RMSD bond angle | 1.334 |
Data reduction software | XDS |
Data scaling software | SCALA (CCP4_3.3.20) |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.580 | 29.580 | 2.270 |
High resolution limit [Å] | 2.150 | 6.800 | 2.150 |
Rmerge | 0.112 | 0.020 | 0.900 |
Total number of observations | 5881 | 20941 | |
Number of reflections | 26687 | ||
<I/σ(I)> | 15.0444 | ||
Completeness [%] | 99.8 | 98.32 | 99.29 |
Redundancy | 6.49 | 6.3 | 5.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 25% PEG-3350, 0.1 M ammonium sulfate, 0.1 M bis-tris. Crystal was soaked for 2 days in a 0.001 M solution of the ligand., pH 5.5, vapor diffusion, sitting drop, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 25% PEG-3350, 0.1 M ammonium sulfate, 0.1 M bis-tris. Crystal was soaked for 2 days in a 0.001 M solution of the ligand., pH 5.5, vapor diffusion, sitting drop, temperature 291K |