3UWB
Crystal structure of a probable peptide deformylase from strucynechococcus phage S-SSM7 in complex with actinonin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-16 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.810, 59.150, 61.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.620 - 1.700 |
R-factor | 0.156 |
Rwork | 0.155 |
R-free | 0.17100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uwa |
RMSD bond length | 0.016 |
RMSD bond angle | 1.703 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.035 | 0.279 |
Number of reflections | 19602 | |
<I/σ(I)> | 5.24 | |
Completeness [%] | 99.2 | 89.2 |
Redundancy | 8.5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 290 | 60.8% MPD, 100MM NAHEPES PH 7.3- 7.4, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |