3UWA
Crystal structure of a probable peptide deformylase from synechococcus phage S-SSM7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791750 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.460, 58.630, 62.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.730 - 1.950 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.20700 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.455 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.088 | 0.484 |
Number of reflections | 13168 | |
<I/σ(I)> | 3.05 | |
Completeness [%] | 99.7 | 95.7 |
Redundancy | 8.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 290 | 60.8% MPD, 100MM NAHEPES PH 7.3- 7.4, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |