3UW6
Crystal Structure of Engineered Protein, Northeast Structural Genomics Consortium Target OR120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.348, 112.348, 237.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.470 - 2.300 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.070 | 0.612 |
| Number of reflections | 85640 | |
| <I/σ(I)> | 23.6 | |
| Completeness [%] | 99.5 | 96.1 |
| Redundancy | 10 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | 1.44M Potassium acetate, 50mM MES, pH 6, Microbatch under oil, temperature 277K |
