3UW1
Crystal structure of ribose-5-phosphate isomerase a from burkholderia thailandensis with ribose-5-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-11 |
Detector | RIGAKU SATURN 944+ |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.420, 73.558, 52.032 |
Unit cell angles | 90.00, 127.97, 90.00 |
Refinement procedure
Resolution | 27.380 - 1.710 |
R-factor | 0.149 |
Rwork | 0.147 |
R-free | 0.17300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.296 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmerge | 0.076 | 0.155 |
Number of reflections | 25863 | |
<I/σ(I)> | 19.9 | |
Completeness [%] | 99.4 | 89.5 |
Redundancy | 7.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 290 | Internal tracking number 225985D11. WIZARD 3/4 screen condition D11: 30%(w/v) PEG 5000 MME, 0.1M MES, 0.2M Ammonium sulfate, ButhA.00944.a.A1 PW33411 at 33.6 mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 290K, pH 6.5 |