3UVU

Structural basis of nuclear import of Flap endonuclease 1 (FEN1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.45
Spacegroup name	P 21 21 21
Unit cell lengths	78.870, 89.561, 100.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.710 - 2.380
R-factor	0.1703
Rwork	0.168
R-free	0.21508
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.023
RMSD bond angle	1.961
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.2.0019)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.443
High resolution limit [Å]	2.380	2.380
Rmerge	0.105	0.105
Number of reflections	28376
<I/σ(I)>		15.04
Completeness [%]	98.0	98
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	Sodium citrate, DTT, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K