3UVU
Structural basis of nuclear import of Flap endonuclease 1 (FEN1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.45 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.870, 89.561, 100.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.710 - 2.380 |
R-factor | 0.1703 |
Rwork | 0.168 |
R-free | 0.21508 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.023 |
RMSD bond angle | 1.961 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.443 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.105 | 0.105 |
Number of reflections | 28376 | |
<I/σ(I)> | 15.04 | |
Completeness [%] | 98.0 | 98 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | Sodium citrate, DTT, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |