3UVH
Crystal Structure Analysis of E81M mutant of human CLIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-10-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.466, 70.096, 82.667 |
| Unit cell angles | 90.00, 90.35, 90.00 |
Refinement procedure
| Resolution | 37.680 - 1.840 |
| R-factor | 0.2036 |
| Rwork | 0.201 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.861 |
| Data reduction software | SAINT (V7.34A) |
| Data scaling software | SAINT (V7.34A) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.680 | 1.912 | |
| High resolution limit [Å] | 1.840 | 3.977 | 1.840 |
| Number of reflections | 41890 | ||
| <I/σ(I)> | 156.715 | ||
| Completeness [%] | 99.6 | ||
| Redundancy | 6.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris, 0.2 M ammonium acetate, 25% (w/v) PEG 3350, 0.02% azide, 5 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
