3UVF
Expanding LAGALIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 181.591, 73.592, 82.031 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.710 - 3.000 |
| R-factor | 0.28488 |
| Rwork | 0.280 |
| R-free | 0.37811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eh8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.004 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.710 | 2.800 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.680 | 2.490 |
| Rmerge | 0.166 | 0.251 | |
| Number of reflections | 22602 | ||
| <I/σ(I)> | 23.2 | 10 | 5.45 |
| Completeness [%] | 97.6 | 96.3 | 93.7 |
| Redundancy | 6.4 | 6.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 25% PEG 3350, 0.2 M Ammonium sulfate, 0.1 M Bis-Tris pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
