3UV0
Crystal structure of the drosophila MU2 FHA domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97947 |
Spacegroup name | P 65 |
Unit cell lengths | 99.860, 99.860, 38.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.870 - 1.900 |
R-factor | 0.1802 |
Rwork | 0.178 |
R-free | 0.22700 |
Structure solution method | MIR |
RMSD bond length | 0.015 |
RMSD bond angle | 1.591 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 20767 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M Calcium acetate, pH 7.0, 18% PEG 3350, 50mM KCl, vapor diffusion, hanging drop, temperature 293K |