3UUX
Crystal structure of yeast Fis1 in complex with Mdv1 fragment containing N-terminal extension and coiled coil domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 174.676, 174.676, 167.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 123.510 - 3.900 |
| R-factor | 0.2709 |
| Rwork | 0.270 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.944 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 123.514 | 19.808 | 4.110 |
| High resolution limit [Å] | 3.896 | 12.330 | 3.900 |
| Rmerge | 0.033 | 0.519 | |
| Total number of observations | 5007 | 13670 | |
| Number of reflections | 11999 | ||
| <I/σ(I)> | 25 | 19.6 | 1.5 |
| Completeness [%] | 98.9 | 76.1 | 98.1 |
| Redundancy | 14.5 | 15.4 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 295 | 0.54M sodium citrate, 0.1M cacodylate, 4% acetonitrile, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
