3UUX

Crystal structure of yeast Fis1 in complex with Mdv1 fragment containing N-terminal extension and coiled coil domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	77
Detector technology	CCD
Collection date	2010-03-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9999
Spacegroup name	I 4 2 2
Unit cell lengths	174.676, 174.676, 167.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	123.510 - 3.900
R-factor	0.2709
Rwork	0.270
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.944
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	123.514	19.808	4.110
High resolution limit [Å]	3.896	12.330	3.900
Rmerge		0.033	0.519
Total number of observations		5007	13670
Number of reflections	11999
<I/σ(I)>	25	19.6	1.5
Completeness [%]	98.9	76.1	98.1
Redundancy	14.5	15.4	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	295	0.54M sodium citrate, 0.1M cacodylate, 4% acetonitrile, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K