3UTW

Crystal structure of bacteriorhodopsin mutant P50A/Y57F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97916
Spacegroup name	C 2 2 21
Unit cell lengths	45.281, 103.059, 128.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.850 - 2.400
R-factor	0.20644
Rwork	0.203
R-free	0.24575
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	2.489
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	11638
Completeness [%]	100.0	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BICELLES, VAPOR DIFFUSION, HANGING DROP	4	310	0.65M sodium phosphate, 0.95% triethylene glycerol, 0.008M 1,6-hexanediol, 4.3% DMPC, 1.5% CHAPSO , BICELLES, VAPOR DIFFUSION, HANGING DROP, temperature 310K