3UTV

Crystal structure of bacteriorhodopsin mutant Y57F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97938
Spacegroup name	C 2 2 21
Unit cell lengths	46.080, 104.009, 129.743
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.070 - 2.060
R-factor	0.19707
Rwork	0.196
R-free	0.21044
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	1.952
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.130
High resolution limit [Å]	2.060	2.060
Number of reflections	19418
Completeness [%]	100.0	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BICELLES, VAPOR DIFFUSION, HANGING DROP	4	310	0.65M sodium phosphate, 0.95% triethylene glycerol, 0.008M 1,6-hexanediol, 4.3% DMPC, 1.5% CHAPSO, BICELLES, VAPOR DIFFUSION, HANGING DROP, temperature 310K