3UTG
Crystal structure of Aspergillus fumigatus UDP galactopyranose mutase complexed with UDP in reduced state
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 218.176, 218.176, 320.822 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.589 - 2.250 |
| R-factor | 0.1934 |
| Rwork | 0.192 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ute |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.589 | 48.589 | 2.370 |
| High resolution limit [Å] | 2.250 | 7.120 | 2.250 |
| Rmerge | 0.017 | 0.676 | |
| Total number of observations | 33545 | 151053 | |
| Number of reflections | 205755 | ||
| <I/σ(I)> | 17.6 | 36.3 | 1.1 |
| Completeness [%] | 97.9 | 90.9 | 99.7 |
| Redundancy | 5 | 5.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 1.5M ammonium sulfate, 0.1M sodium acetate, 5mM L-cysteine, 0.5mM THP , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
