3UTB

Crystal Structure of Nucleosome Core Particle Assembled with the 146b Alpha-Satellite Sequence (NCP146b)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Detector technology	PIXEL
Detector	PSI PILATUS 6M
Wavelength(s)	1.07
Spacegroup name	P 21 21 21
Unit cell lengths	105.460, 109.280, 175.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.460 - 2.200
R-factor	0.233
Rwork	0.232
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.387
Data scaling software	SCALA (3.3.15)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	92.828	58.640	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge		0.034	0.484
Rmeas		0.043	0.634
Rpim		0.021	0.318
Total number of observations		14458	48222
Number of reflections	101720
<I/σ(I)>	13.3	31.1	2.2
Completeness [%]	98.3	98.3	89.9
Redundancy	5.3	4.2	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	K-cacodylate, KCl, MnCl2, pH 6.0, temperature 291K, VAPOR DIFFUSION, HANGING DROP