3UTA
Crystal Structure of Nucleosome Core Particle Assembled with an Alpha-Satellite Sequence Containing Two TTAAA elements (NCP-TA2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.510, 109.910, 182.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 94.130 - 2.070 |
R-factor | 0.2411 |
Rwork | 0.240 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.418 |
Data scaling software | SCALA (3.3.16) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 94.130 | 106.510 | 2.180 |
High resolution limit [Å] | 2.067 | 6.540 | 2.070 |
Rmerge | 0.063 | 0.452 | |
Rmeas | 0.077 | 0.079 | 0.601 |
Rpim | 0.035 | 0.037 | 0.411 |
Total number of observations | 413096 | 18774 | 4412 |
Number of reflections | 99095 | ||
<I/σ(I)> | 12.1 | 20.6 | 1.4 |
Completeness [%] | 75.7 | 99.3 | 15.4 |
Redundancy | 4.2 | 4.2 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | K-cacodylate, KCl, MnCl2, pH 6.0, temperature 291K, VAPOR DIFFUSION, HANGING DROP |