3UT5
Tubulin-Colchicine-Ustiloxin: Stathmin-like domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.810, 128.870, 254.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.260 - 2.730 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.020 | 2.870 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.102 | 0.568 |
| Number of reflections | 56466 | |
| <I/σ(I)> | 14.8 | 1.7 |
| Completeness [%] | 97.3 | 81.7 |
| Redundancy | 7.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 293 | PEG, LISO4, PIPES BUFFER, PH 6.80, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
