3USY

Crystal structure of Flig (residue 116-343) from H. Pylori

Replaces:  3PL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	85.378, 102.564, 91.582
Unit cell angles	90.00, 114.69, 90.00

Refinement procedure

Resolution	29.200 - 2.706
R-factor	0.1966
Rwork	0.194
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lkv
RMSD bond length	0.009
RMSD bond angle	1.084
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.200
High resolution limit [Å]	2.700
Number of reflections	19547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.1M HEPES, 10% PEG6000, 0.02M SPERMIDINE, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K