3USQ
Structure of D159S/Y194F glycogenin mutant truncated at residue 270
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-14 |
Detector | MARRESEARCH |
Wavelength(s) | 1.430 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 56.530, 105.640, 120.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.320 - 2.400 |
R-factor | 0.21512 |
Rwork | 0.213 |
R-free | 0.25919 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.307 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.320 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.068 | 0.397 |
<I/σ(I)> | 1.9 | 1.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12% PEGMME5000/0.2M ammonium sulfate/sodium MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |