3USL
Crystal Structure of LeuT bound to L-selenomethionine in space group C2 from lipid bicelles
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.323, 90.062, 81.968 |
Unit cell angles | 90.00, 103.88, 90.00 |
Refinement procedure
Resolution | 37.156 - 2.710 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.24430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a65 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.647 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.810 |
High resolution limit [Å] | 2.710 | 2.710 |
Rmerge | 0.080 | 0.340 |
Number of reflections | 23553 | |
<I/σ(I)> | 12.2 | 3.4 |
Completeness [%] | 98.8 | 92.2 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 100 mM NaAc, 35% MPD, 10%PEG, 50 mM MgCl2, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |