3USH
Crystal Structure of the Q2S0R5 protein from Salinibacter ruber, Northeast Structural Genomics Consortium Target SrR207
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.079, 85.417, 42.800 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 30.987 - 1.692 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.402 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.246 |
| Number of reflections | 48111 | |
| <I/σ(I)> | 23.4 | 4.4 |
| Completeness [%] | 98.4 | 97.9 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 6 | 291 | 40% PEG 400, 0.1M potassium bromide, 0.1M MES, pH 6.0, Microbatch crystallization under oil, temperature 291K |
