3US4

Crystal structure of a SH2 domain of a megakaryocyte-associated tyrosine kinase (MATK) from Homo sapiens at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9999,0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	63.840, 57.560, 28.750
Unit cell angles	90.00, 116.20, 90.00

Refinement procedure

Resolution	25.593 - 1.500
R-factor	0.1866
Rwork	0.185
R-free	0.20810
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.593	25.593	1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge	0.047	0.036	0.481
Number of reflections	14591	132	1036
<I/σ(I)>	16.87	25.5	3.29
Completeness [%]	96.9	71.7	93.6
Redundancy	5.02		4.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	2.40M (NH4)2SO4, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K