3UR8
Lower-density crystal structure of potato endo-1,3-beta-glucanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-02-04 |
Detector | MAR555 FLAT PANEL |
Wavelength(s) | 0.8148 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.361, 49.056, 82.618 |
Unit cell angles | 90.00, 103.56, 90.00 |
Refinement procedure
Resolution | 19.540 - 1.260 |
R-factor | 0.14277 |
Rwork | 0.142 |
R-free | 0.18244 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ur7 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.744 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.310 |
High resolution limit [Å] | 1.260 | 1.260 |
Rmerge | 0.059 | 0.467 |
Number of reflections | 151662 | |
<I/σ(I)> | 19.2 | 2 |
Completeness [%] | 95.4 | 93.8 |
Redundancy | 4.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | 0.1 M sodium acetate, 0.2 M ammonium acetate, 25% PEG 4000, 15 mM glucose, streak seeding, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |