3UR7
Higher-density crystal structure of potato endo-1,3-beta-glucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.04300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.128, 49.133, 80.504 |
| Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.16153 |
| Rwork | 0.161 |
| R-free | 0.18590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ghs |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.731 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.071 | 0.490 |
| Number of reflections | 110982 | |
| <I/σ(I)> | 20.1 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | 0.1 M sodium acetate, 0.2 M ammonium acetate, 25% PEG 4000, 15 mM glucose, streak seeding, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
