3UQZ

X-ray structure of DNA processing protein A (DprA) from Streptococcus pneumoniae

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9330
Spacegroup name	I 41 2 2
Unit cell lengths	300.240, 300.240, 78.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.760 - 2.700
R-factor	0.2206
Rwork	0.219
R-free	0.24510
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXS
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	47450
<I/σ(I)>		3.1
Completeness [%]	95.9	92.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1M MES pH 6.5, 1M LiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1M MES pH 6.5, 1M LiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K