3UQN
Crystal structure of dihydrodipicolinate synthase from Acinetobacter baumannii complexed with Oxamic acid at 1.9 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-09-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.374, 122.637, 52.332 |
Unit cell angles | 90.00, 115.46, 90.00 |
Refinement procedure
Resolution | 61.320 - 1.940 |
R-factor | 0.17995 |
Rwork | 0.179 |
R-free | 0.19836 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rk8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.539 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.320 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Number of reflections | 45562 | |
<I/σ(I)> | 8.8 | 2 |
Completeness [%] | 99.1 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1M MgCl2, PEG3350, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |