Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.466, 80.466, 124.929 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.510 - 3.000 |
| R-factor | 0.22564 |
| Rwork | 0.219 |
| R-free | 0.28802 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kdh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.690 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.134 | 0.521 |
| Number of reflections | 9652 | |
| <I/σ(I)> | 12.7 | 3.2 |
| Completeness [%] | 98.2 | 99.4 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 25% PEG 3350, 0.1M BIS-TRIS PH 6.5, 0.2M (NH4)2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
