3UQE
Crystal structure of the Phosphofructokinase-2 mutant Y23D from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 43.838, 89.123, 175.908 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.980 - 2.200 |
R-factor | 0.22 |
Rwork | 0.219 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cqd |
RMSD bond length | 0.012 |
RMSD bond angle | 0.927 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 58.620 |
High resolution limit [Å] | 2.200 |
Number of reflections | 33605 |
Completeness [%] | 94.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 291 | 23% PEG 4000, 0.1 M SODIUM ACETATE PH 4.75, 0.2 M AMMONIUM ACETATE, VAPOR DIFFUSION, HANGING DROP, temperature 291K |