3UQC
Structure of the Intracellular Kinase Homology Domain of Rv3910 at 2.2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.115872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.974, 59.382, 144.031 |
Unit cell angles | 90.00, 97.34, 90.00 |
Refinement procedure
Resolution | 47.877 - 2.256 |
R-factor | 0.2015 |
Rwork | 0.199 |
R-free | 0.24020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ouk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.991 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (- Phaser) |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.891 | 2.330 |
High resolution limit [Å] | 2.256 | 2.250 |
Number of reflections | 46032 | |
<I/σ(I)> | 15.7 | 3 |
Completeness [%] | 99.3 | 93.5 |
Redundancy | 4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.7-0.8 M succinate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |