3UQ5
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240A F247L (L9A F16L) in the presence of 10 mM cysteamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.570, 267.430, 111.160 |
Unit cell angles | 90.00, 108.64, 90.00 |
Refinement procedure
Resolution | 19.988 - 4.200 |
R-factor | 0.2213 |
Rwork | 0.220 |
R-free | 0.24940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vl0 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.638 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_897) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.988 | 4.310 | |
High resolution limit [Å] | 4.200 | 18.780 | 4.200 |
Rmerge | 0.179 | 0.023 | 0.829 |
Number of reflections | 42057 | 77 | 3139 |
<I/σ(I)> | 16.58 | 82.16 | 4.17 |
Completeness [%] | 98.7 | 15.4 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10-12% PEG4000, 200 mM ammonium sulfate, 50 mM N-(2-acetamido)iminodiacetic acid (ADA), pH 6.5-6.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |