3UPU
Crystal structure of the T4 Phage SF1B Helicase Dda
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 77.2 |
| Detector technology | CCD |
| Collection date | 2009-06-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 225.536, 107.253, 85.475 |
| Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
| Resolution | 32.271 - 3.299 |
| R-factor | 0.2065 |
| Rwork | 0.204 |
| R-free | 0.25760 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.916 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.035 | 3.420 |
| High resolution limit [Å] | 3.299 | 3.299 |
| Rmerge | 0.092 | 0.473 |
| Number of reflections | 29389 | |
| <I/σ(I)> | 19.82 | 2.05 |
| Completeness [%] | 95.8 | 71.4 |
| Redundancy | 7.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.1 | 4.5% w/v PEG8000, 8% v/v ethylene glycol, 0.1 M HEPES, pH 6.5, 20 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291.1K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.1 | 4.5% w/v PEG8000, 8% v/v ethylene glycol, 0.1 M HEPES, pH 6.5, 20 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291.1K |
