3UOZ

Crystal Structure of OTEMO complex with FAD and NADP (form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-18
Detector	RAYONIX MX225HE
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	66.762, 95.179, 93.130
Unit cell angles	90.00, 101.82, 90.00

Refinement procedure

Resolution	48.607 - 2.407
R-factor	0.1989
Rwork	0.196
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uoy
RMSD bond length	0.014
RMSD bond angle	1.576
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.157	91.157	2.510
High resolution limit [Å]	2.407	5.210	2.407
Rmerge	0.105	0.048	0.452
Number of reflections	42173
<I/σ(I)>	8.8
Completeness [%]	95.8	95.4	91
Redundancy	2.6	2.7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	22% PEG3350, 0.1 M sodium/potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K