3UO3

Jac1 co-chaperone from Saccharomyces cerevisiae, 5-182 clone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	59.791, 60.450, 108.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.500 - 1.850
R-factor	0.1943
Rwork	0.192
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uo2
RMSD bond length	0.017
RMSD bond angle	1.714
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.500	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.071	0.042	0.791
Number of reflections	34454
<I/σ(I)>	11		2.21
Completeness [%]	99.9	99.2	99.7
Redundancy	13.5	13	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.085 M Tris-HCl buffer, 0.17 M sodium acetate, 25.5% PEG-4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K