3UO2
Jac1 co-chaperone from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-13 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.606, 60.548, 99.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.100 - 2.130 |
R-factor | 0.213 |
Rwork | 0.210 |
R-free | 0.27330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bvo |
RMSD bond length | 0.016 |
RMSD bond angle | 1.747 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.100 | 50.000 | 2.170 |
High resolution limit [Å] | 2.130 | 5.780 | 2.130 |
Rmerge | 0.064 | 0.036 | 0.894 |
Number of reflections | 20799 | ||
<I/σ(I)> | 12.5 | 4.7 | |
Completeness [%] | 99.8 | 96 | 100 |
Redundancy | 22.9 | 19.7 | 23.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES buffer, 0.2 M ammonium acetate, 25% PEG-3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |