3UNO
Mycobacterium tuberculosis ferritin homolog, BfrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 226.234, 226.750, 113.678 |
| Unit cell angles | 90.00, 94.75, 90.00 |
Refinement procedure
| Resolution | 49.352 - 2.503 |
| R-factor | 0.21 |
| Rwork | 0.206 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.249 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 19297 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 11.9%(w/v) PEG MME 5000, 100 mM Tris maleate, 0.54%(w/v) 1,6-hexanediol, 0.44%(w/v) lauryldimethylamine oxide, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
