3UNM
Crystal Structure of The Human MDC1 FHA Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.651, 56.053, 64.920 |
| Unit cell angles | 90.00, 97.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.2365 |
| Rwork | 0.234 |
| R-free | 0.27410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3umz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.241 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.863 | 1.800 |
| Rmerge | 0.079 | 0.045 | 0.448 |
| Number of reflections | 20009 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 97.7 | 77.2 | 91.2 |
| Redundancy | 3.4 | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 3350, 0.1M HEPES (pH 7.5), 50mM KCl, vapor diffusion, hanging drop, temperature 293K |
