3UNH
Mouse 20S immunoproteasome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.280, 205.220, 161.940 |
| Unit cell angles | 90.00, 105.70, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.200 |
| R-factor | 0.23938 |
| Rwork | 0.239 |
| R-free | 0.25395 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iru |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.851 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.104 | 0.515 |
| Number of reflections | 121329 | |
| <I/σ(I)> | 10.4 | 2.3 |
| Completeness [%] | 99.2 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.2 M NaI, 40% MPD, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
