3UMV
Eukaryotic Class II CPD photolyase structure reveals a basis for improved UV-tolerance in plants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979764 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.351, 109.362, 97.742 |
Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
Resolution | 45.555 - 1.705 |
R-factor | 0.1816 |
Rwork | 0.181 |
R-free | 0.21660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1DNP 1qnf and 1IQR |
RMSD bond length | 0.006 |
RMSD bond angle | 1.052 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.056 | 0.341 |
Number of reflections | 119326 | |
<I/σ(I)> | 23.1 | 2.87 |
Completeness [%] | 98.5 | 98 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 35% PEG 4K, 5% saturated Urea, 200 mM immidazole malate , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |