3UMO
Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Potassium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-09-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97627 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 43.812, 88.770, 176.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.967 - 1.696 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.20790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cqd |
RMSD bond length | 0.011 |
RMSD bond angle | 1.297 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.800 |
High resolution limit [Å] | 1.696 | 5.060 | 1.696 |
Rmerge | 0.051 | 0.026 | 0.590 |
Number of reflections | 76251 | 3105 | 12064 |
<I/σ(I)> | 15.06 | 40.58 | 2.04 |
Completeness [%] | 98.8 | 97.6 | 98.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 291 | 23% PEG 4000, 0.1 M SODIUM ACETATE PH 4.75, 0.2 M AMMONIUM ACETATE, vapor diffusion, hanging drop, temperature 291K |