3UMD
Structure of pB intermediate of Photoactive yellow protein (PYP) at pH 4.
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 - 1.2 |
| Spacegroup name | P 63 |
| Unit cell lengths | 66.900, 66.900, 40.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| Rwork | 0.196 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2phy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.513 |
| Data reduction software | XFIT |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 10000 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 3 M Ammonium Sulfate, 50 mM Sodium Phosphate, pH 4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
