3ULX

Crystal structural of the conserved domain of Rice Stress-responsive NAC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	45.972, 45.972, 176.494
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.507 - 2.600
R-factor	0.2286
Rwork	0.227
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ut4
RMSD bond length	0.011
RMSD bond angle	1.547
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	6435
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M HEPES, 1.5M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K