3ULR
Lysozyme contamination facilitates crystallization of a hetero-trimericCortactin:Arg:Lysozyme complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.658, 57.838, 95.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.650 |
R-factor | 0.18641 |
Rwork | 0.184 |
R-free | 0.22487 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2D1X and 3M3U |
RMSD bond length | 0.019 |
RMSD bond angle | 1.771 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 23866 | |
<I/σ(I)> | 14.9 | 2.5 |
Completeness [%] | 95.0 | 65.8 |
Redundancy | 5.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 297 | 1.0M Na Citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |